Microwave gallium-68 radiochemistry for kinetically stable bis(thiosemicarbazone) complexes: structural investigations and cellular uptake under hypoxia† †Electronic supplementary information (ESI) available. CCDC 1001632–1001634. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5dt02537k Click here for additional data file. Click here for additional data file.

5. Radiochemistry 24 6. Laser scanning confocal microscopy 30 7. Two-photon excitation and fluorescence lifetime imaging microscopy 44 8. Kinetic stability testing in an aqueous environment 50 9. Stability assays to estimate kinetic stability against common biologically-relevant agents 55 10. In vivo imaging tests by microPET 62 11. Hypoxia selectivity testing 63 12. MTT assays 69 13. Cyclic voltammetry experiment of bis(thiosemicarbazonato) complexes and ligands 72 14. References 76 3 1. General Information All reagents were purchased from Sigma-Aldrich, Alfa-Aesar, Merck Chemicals or Acros Organics and were used as received unless otherwise stated. 1 H NMR spectra were recorded on a Bruker Avance spectrometer (300 MHz, 400 MHz) or a Bruker Avance II+ (500 MHz) spectrometer at 298 K. Chemical shifts δ in ppm were referenced to the solvent residual peak as an internal standard. spectrometer (300 MHz, 400 MHz) or a Bruker Avance II+ (500 MHz) and spectra were referenced to the solvent residual peak. UV-visible spectra were obtained using a Perkin-Elmer Spectrometer, Lamda 650 in DMSO and processed using UV Winlab 3 software; the orientation of the 1.00 cm quartz cuvette was the same for each experiment for consistency. Fluorescence spectra were measured in a 1.00 cm quartz cuvette using a Perkin-Elmer LS55 luminescence spectrophotometer; a scan from 200-800nm with increments of 10 nm was initially carried out to discover excitation wavelength of maximum emission (λ ex-max) and wavelength of maximum emission (λ em-max). Elemental analysis were carried out